Selection of NAT-Resistant Yeast Transformants: Isolates Saccharomyces cerevisiae colonies carrying NatMX or clonNAT-resistance cassettes on rich YPD (1.0% yeast extract, 2.0% peptone, 2.0% glucose, 2.0% agar) with 100 µg/mL ClonNAT.

Plasmid Maintenance and Recovery: Maintains yeast plasmids bearing the NAT selectable marker and enables recovery of individual clones.

Genome Editing and Integration Screening: Selects successful CRISPR/Cas9 edits or genomic integrations using NAT-marked donor cassettes.

Yeast Genetic Crosses: Selects haploids or diploids carrying NAT-marked alleles after mating, sporulation, or tetrad dissection.

Replica Plating and Strain Purification: Confirms genotype by transferring colonies onto NAT plates to verify marker retention and eliminate revertants.

Library and Fitness Screens: Enables pooled or arrayed yeast library selection (e.g., barcoded or deletion collections) under NAT pressure.

Colony Isolation and CFU Enumeration: Counts viable NAT-resistant yeast and isolates single colonies for downstream assays.

Competitive Growth Assays: Compares growth of NAT-tagged strains versus controls on rich medium with 100 µg/mL ClonNAT.

Strain Archiving and Maintenance: Short-term storage and routine passaging of NAT-selected clones on robust YPD.

Quality Control of Constructs: Verifies retention of NAT markers after transformations, streak-purification, or propagation.