AEX Process Development for AAV8: Rapid screening of equilibration and elution buffers to establish binding and elution conditions specific to AAV8.

Buffer Scouting (pH and Conductivity): Optimization of pH and salt gradients to maximize recovery and purity across multiple AEX conditions.

Empty/Full Capsid Enrichment: Tuning AEX conditions to improve separation of empty from full AAV8 capsids.

Impurity Clearance: Removal of host-cell proteins, host-cell DNA, and process-related impurities during polishing.

Scale-Down DOE and Method Development: Design-of-experiments screening in small scale to define robust operating ranges before scale-up.

Resin and Column Comparability: Evaluate performance across different AEX resins/columns using matched buffer sets.

Robustness and Transfer Studies: Assess method ruggedness and support tech transfer between labs and manufacturing sites.

Troubleshooting and Optimization: Systematic adjustment of equilibration/elution conditions to resolve breakthrough or poor resolution.

High-Throughput Screening: Compatible with automated platforms for rapid parallel AEX condition testing.

Pre-Scale-Up Feasibility: Confirm AEX viability and define platform conditions prior to larger batch purification.