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M9 Agar Plates

Our M9 agar plates are pre-poured, fully defined, minimal-growth media for culturing bacteria. M9 minimal media is widely used in microbiology and molecular biology applications to study bacterial metabolism, nutrient utilization, and gene regulation under controlled growth conditions. Our extensive collection of M9 agar plates is formulated with various carbon sources, additives, and antibiotics to meet a wide range of R&D or manufacturing needs.
M9 Agar Plates
What are M9 agar plates?

M9 agar plates are a defined, minimal solid growth medium composed of M9 salts, agar, and a carbon source such as glucose. Unlike rich media, M9 agar provides essential inorganic salts and nutrients, allowing precise control over bacterial growth conditions and metabolic inputs.

 

M9 agar plates are commonly used for cultivating E. coli and other non-fastidious bacteria in applications including metabolic and physiological studies, auxotrophic strain analysis, and genetic screening or selection. Because M9 agar is a minimal medium, it is particularly valuable for experiments that require controlled nutrient composition or selective growth based on metabolic capability.

 

M9 agar plates may be supplemented with defined carbon sources, amino acids, vitamins, or antibiotics to support your specific workflow or experimental objectives.

Why choose Teknova's M9 agar plates?

Our M9 agar plates are supplied as ready-to-use pre-poured plates, eliminating in-house media preparation and reducing variability. Formulated as a defined, minimal medium, our M9 agar plates provide controlled growth conditions and are well-suited for studying bacterial metabolism, selection, and physiology. The plates can also be supplemented with carbon sources, amino acids, or antibiotics to support specific experimental needs.


We manufacture all our M9 agar plates using validated processes and rigorous quality control to ensure growth performance and lot-to-lot consistency. In addition to our extensive catalog of ready-to-ship products, we provide industry-leading turnaround times for custom manufacturing, with custom options including modified M9 formulations, alternative carbon sources, antibiotic combinations, and plate formats to support specialized microbiology workflows.

Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Frequently asked questions

You can find answers here for our most commonly asked questions. If you don't find the information you are looking for, get in touch with our team. We're here to help.

M9 agar plates are a defined, minimal growth medium used to culture bacteria under controlled nutrient conditions. They are commonly used for studying bacterial metabolism, auxotrophy, and physiology.

M9 agar plates primarily support the growth of non-fastidious bacteria, including laboratory strains of E. coli. Growth depends on the availability of an appropriate carbon source and any required additives for auxotrophic strains.

M9 agar plates are a defined minimal medium containing only essential salts and nutrients, whereas LB agar plates are a rich medium containing yeast extract and tryptone. M9 agar allows tighter control of nutrient availability and is preferred for metabolic and genetic studies.

Yes. M9 agar plates can be supplemented with various carbon sources, such as glucose, amino acids, vitamins, or antibiotics, to support specific bacterial growth requirements. We sell M9 agar plates supplemented with thiamine (0.2 µg/mL), glucose (0.2%, 0.4%, and 1%), magnesium sulfate (1 mM), uracil (250 µg/mL), kanamycin (50 µg/mL), and proline (250 µg/mL). You can also start a custom order to get a formulation made to your specifications.

Most of our M9 agar plates can be stored at room temperature, but those containing antibiotics should be refrigerated. The storage instructions and shelf life for your specific product can be found on your product label.

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

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