M9 Agar Plates
M9 agar plates are a defined, minimal growth medium used to culture bacteria under controlled nutrient conditions. They are commonly used for studying bacterial metabolism, auxotrophy, and physiology.
M9 agar plates primarily support the growth of non-fastidious bacteria, including laboratory strains of E. coli. Growth depends on the availability of an appropriate carbon source and any required additives for auxotrophic strains.
M9 agar plates are a defined minimal medium containing only essential salts and nutrients, whereas LB agar plates are a rich medium containing yeast extract and tryptone. M9 agar allows tighter control of nutrient availability and is preferred for metabolic and genetic studies.
Yes. M9 agar plates can be supplemented with various carbon sources, such as glucose, amino acids, vitamins, or antibiotics, to support specific bacterial growth requirements. We sell M9 agar plates supplemented with thiamine (0.2 µg/mL), glucose (0.2%, 0.4%, and 1%), magnesium sulfate (1 mM), uracil (250 µg/mL), kanamycin (50 µg/mL), and proline (250 µg/mL). You can also start a custom order to get a formulation made to your specifications.
Most of our M9 agar plates can be stored at room temperature, but those containing antibiotics should be refrigerated. The storage instructions and shelf life for your specific product can be found on your product label.
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