We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

Bis-Tris Solutions

Our Bis-Tris buffer solutions are formulated for electrophoresis and biochemical workflows that require precise pH control near neutrality. Bis-Tris buffer is widely used in SDS-PAGE, agarose gel electrophoresis, protein purification, and biochemistry applications.
Bis-Tris Solutions
What are Bis-Tris buffer solutions?

Bis-Tris buffer (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol) is a zwitterionic biological buffer with an effective buffering range of approximately pH 5.8-7.2, making it well suited for applications near physiological pH. Compared to Tris buffer, Bis-Tris exhibits reduced temperature- and concentration-dependent pH shifts and minimal interaction with divalent metal ions.

 

Bis-Tris buffer solutions are commonly used in Bis-Tris gel electrophoresis systems, protein separation workflows, enzyme activity assays, and general biochemical applications where consistent pH control is essential. We offer ready-to-use Bis-Tris buffer solutions to eliminate in-house preparation and reduce variability in sensitive workflows.

Why choose Teknova’s Bis-Tris buffer solutions?

Our Bis-Tris buffer solutions are supplied as ready-to-use formulations, saving time and minimizing the variability associated with manual buffer preparation. Each Bis-Tris buffer solution is manufactured to precise specifications to ensure reliable buffering performance across applications.


Our Bis-Tris buffers are designed to integrate seamlessly into routine laboratory operations, from protein electrophoresis and analytical biochemistry to method development and quality control testing. All products are manufactured under rigorous quality control standards to ensure pH accuracy, purity, and lot-to-lot consistency. We also offer custom Bis-Tris buffer solutions, including modified concentrations, adjusted pH values, and alternative container formats to meet specialized research or manufacturing requirements.

Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Frequently asked questions

You can find answers here for our most commonly asked questions. If you don't find the information you are looking for, get in touch with our team. We're here to help.

Bis-Tris buffer is commonly used in DNA and protein electrophoresis, enzyme assays, and biochemical workflows that require stable pH control near neutrality. It is particularly popular in Bis-Tris gel systems for protein separation.

Bis-Tris buffer has an effective buffering range of approximately pH 5.8 to 7.2, making it suitable for applications near physiological pH. For more details, see our guide to optimal pH.

Compared to Tris buffer, Bis-Tris buffer exhibits smaller pH changes with temperature fluctuations and reduced interaction with divalent metal ions, providing more stable buffering under certain experimental conditions.

Our Bis-Tris buffer solutions should be stored at room temperature. The storage instructions and shelf life for your specific product can be found on your product label.

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button