Bis-Tris Solutions
Bis-Tris buffer is commonly used in DNA and protein electrophoresis, enzyme assays, and biochemical workflows that require stable pH control near neutrality. It is particularly popular in Bis-Tris gel systems for protein separation.
Bis-Tris buffer has an effective buffering range of approximately pH 5.8 to 7.2, making it suitable for applications near physiological pH. For more details, see our guide to optimal pH.
Compared to Tris buffer, Bis-Tris buffer exhibits smaller pH changes with temperature fluctuations and reduced interaction with divalent metal ions, providing more stable buffering under certain experimental conditions.
Our Bis-Tris buffer solutions should be stored at room temperature. The storage instructions and shelf life for your specific product can be found on your product label.
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