Brain Heart Infusion (BHI) broth is a nonselective microbiological medium formulated from infusions of brain and heart tissue, combined with peptones, dextrose, disodium phosphate, and sodium chloride. This composition supplies essential amino acids, peptides, carbohydrates, vitamins, and other growth factors required for the cultivation of fastidious and non-fastidious microbes, including bacteria, yeasts, and molds. BHI can be supplemented with blood, additional carbon sources, or antimicrobial agents for specific purposes.

BHI broth is commonly used for the cultivation and maintenance of streptococci, pneumococci, Listeria species, Neisseria species, and other clinically relevant microorganisms.

Our BHI broth is supplied as a sterile, ready-to-use liquid medium, eliminating preparation time and reducing variability in microbiology workflows. We offer a wide selection of BHI broth formulations that are optimized to support consistent microbial growth in suspension cultures for QC, environmental monitoring, and research applications.

We manufacture BHI broth using validated production processes and quality control to ensure lot-to-lot consistency and dependable performance, from routine culturing and strain maintenance to method development and quality testing.

Get BHI broth products in a wide variety of ready-to-use formats and formulations, with additions including yeast extract and L-cysteine. If you don’t see the BHI broth formulation you need, we can manufacture a custom product for you, including specialized formulations, fill volumes, antibiotic combinations, and container formats tailored to your application requirements.