Selection of NAT-resistant yeast transformants: Isolates Saccharomyces cerevisiae carrying natMX/NAT1 markers after transformation.

Plasmid/genome integration maintenance: Propagates strains harboring NAT-marked plasmids or genomic insertions.

CRISPR/Cas9 editing selection: Selects edited clones using natMX-based donor cassettes.

Yeast two-hybrid workflows: Maintains/selects Y2H strains when vectors or reporters use NAT resistance.

Colony isolation and purification: Streaks for single colonies and purifies NAT-resistant yeast lines.

Replica plating and genotype verification: Screens libraries or segregants on NAT to confirm marker presence.

CFU enumeration and viability checks: Quantifies viable NAT-resistant yeast under rich growth conditions.

Combinatorial selection: Used with additional antibiotics (e.g., G418, HygB) to select multi-marked strains.

Phenotypic screening under selection: Assesses growth phenotypes of NAT-marked mutants on rich medium.

Short-term strain maintenance: Routine passaging and storage of NAT-resistant yeast on 100 mm plates.