Selection of Zeocin-resistant yeast transformants: Isolating and maintaining Saccharomyces cerevisiae and Pichia pastoris (Komagataella phaffii) clones carrying Sh ble-based Zeocin® resistance.

Recombinant protein expression screening in yeast: Screening and stabilizing expression clones in S. cerevisiae or P. pastoris on rich medium with 250 µg/mL Zeocin®.

CRISPR/Cas9-edited yeast selection: Selecting edited colonies harboring Zeocin® resistance cassettes after genome engineering.

Vector maintenance and integration verification: Selecting integrative or episomal yeast shuttle vectors bearing Sh ble.

Colony isolation and replica plating: Picking single colonies and replica plating under Zeocin® pressure to verify stability.

Transformation efficiency and titering: Quantifying CFUs of Zeocin®-resistant yeast after transformation or electroporation.

Pichia pastoris expression strain workflows: Plate-based selection and maintenance for AOX1 and other systems using Zeocin® markers.

Library construction and screening in yeast: Maintaining and selecting pooled libraries (e.g., cDNA, mutant, or gRNA libraries) with Zeocin® resistance.

Contamination control during yeast enrichment: Suppressing sensitive microbial contaminants, including Escherichia coli and Staphylococcus aureus, while enriching resistant yeast.

Strain banking and stability testing: Periodic verification of Zeocin®-marked yeast strains for plasmid/integration stability on plates.