Selection of Carbenicillin-Resistant Clones: Growth and selection of Escherichia coli harboring β-lactam–resistant plasmids using 100 µg/mL carbenicillin.

High-Density Colony Growth: Rich 2xYT formulation supports robust colony formation for cloning and screening workflows.

Plasmid Maintenance and Amplification: Stable maintenance and amplification of ampicillin/carbenicillin-selectable plasmids in E. coli.

Reduced Leaky Expression: 2% glucose represses lac-based promoters, minimizing toxic protein expression during clone recovery.

Phage Display Workflows: Commonly used for M13 phagemid libraries (2xYT + glucose + carbenicillin) prior to helper phage infection.

Transformation Plating: Post-chemical or electroporation plating for efficient recovery and selection of transformants.

Colony Picking and Screening: Colony PCR, restriction analysis, and Sanger sequencing template preparation from selected colonies.

Starter Culture Initiation: Picking isolated colonies into liquid media for minipreps and downstream protein expression.

Library Screening: Plating and selection of combinatorial plasmid libraries in E. coli.

High-Throughput Automation: Large 245 mm low-profile format enables automated colony picking and trackable arraying.