Selection and Maintenance of Ampicillin-Resistant Clones: Isolation and propagation of transformed Escherichia coli carrying Amp-resistant plasmids.

Molecular Cloning and Plasmid Propagation: High-yield colony growth on 2xYT (1.6% Tryptone, 1.0% Yeast Extract, 0.5% NaCl, 1.5% Agar) with 2.0% glucose and 100 µg/mL ampicillin for stable plasmid maintenance.

Colony Isolation and Picking: Efficient recovery of single colonies for downstream minipreps, PCR screening, and sequencing.

CFU Enumeration and Viability Testing: Quantification of ampicillin-resistant E. coli populations on selective solid media.

Library Construction and Screening: Plating of plasmid or genomic libraries under ampicillin selection for clone recovery and amplification.

Expression Workflow Support: Glucose-supplemented medium reduces basal expression from lac-based promoters during cloning steps in E. coli.

Phage/Phagemid Workflows: Plating of infected E. coli carrying ampicillin-selectable phagemids for titering and clone recovery.

Strain Banking and Stability Checks: Short-term storage and stability assessment of Amp-resistant E. coli strains on selective plates.