Plasmid Selection and Maintenance: Selects for AmpR/CarbR plasmids in Escherichia coli using 100µg/mL carbenicillin on a rich 2×YT glucose agar base.

High-Density Colony Screening: Large 245mm format enables high-throughput plating, colony counting, and automated picking of Escherichia coli transformants.

Library Plating: Supports transformation and screening of cDNA, shRNA, and CRISPR libraries in Escherichia coli with beta-lactam selection.

Basal Expression Suppression: 2% glucose reduces leaky expression from lac/T7 systems prior to induction in protein expression strains of Escherichia coli.

Blue-White Screening (with additives): Compatible with X-Gal/IPTG supplementation for alpha-complementation on carbenicillin plates in Escherichia coli.

Colony PCR and Sequencing Prep: Rapid isolation of correctly selected Escherichia coli clones for downstream PCR and Sanger/NGS workflows.

Phagemid and Filamentous Phage Workflows: Selection of AmpR phagemid-containing Escherichia coli during M13 rescue and display library steps.

Bacterial Lawns and Spot Assays: Uniform lawns of selected Escherichia coli for spot tests, drop plating, and titer comparisons.

Beta-Lactamase Construct Validation: Screening and verification of bla-containing vectors and promoter variants in Escherichia coli.

Transformation Efficiency and QC: Benchmarking competent cells and workflow performance via selectable colony yield on 2×YT glucose carbenicillin plates.