Dual-Antibiotic Selection of Escherichia coli Transformants: Selects for plasmids carrying beta-lactamase (Carbenicillin 100 µg/mL) and tetracycline resistance (20 µg/mL) on rich 2×YT (1.6% tryptone, 1% yeast extract, 0.5% NaCl) with 2% glucose and 2% agar.

Co-Transformation and Co-Maintenance of Dual-Plasmid Systems: Ensures retention of two compatible plasmids (CarbR + TetR) in E. coli for cloning, assembly, and pathway construction.

Reduced Basal Expression for Toxic or Burdensome Constructs: 2% glucose represses lac/T7-lac promoters, minimizing leaky expression in E. coli prior to induction.

Phagemid/Phage Display Workflows: Supports selection of CarbR phagemids and TetR helper/F′-associated elements in E. coli on rich 2×YT with glucose to control expression during phage production.

High-Density Colony Screening on 245 mm Plates: Large-format plates enable high-throughput colony isolation, replica plating, and automated picking under dual selection.

Verification of Recombinant Constructs Prior to Expression: Confirms plasmid integrity and antibiotic markers in E. coli before liquid culture and induction.

Library Construction and Maintenance: Supports combinatorial DNA libraries in E. coli with stable selection for two markers and suppressed background expression via glucose.

CRISPR/Cas and Gene Editing Workflows: Maintains multi-vector systems (e.g., editor + guide plasmid) in E. coli using Carbenicillin and Tetracycline selection.

Conjugation and Mating Selections: Dual selection of transferred plasmids in recipient E. coli strains on rich, glucose-supplemented 2×YT agar.

Transformation Efficiency and Strain QC: Assesses transformation performance and antibiotic resistance phenotypes in E. coli under Carb100/Tet20 conditions.