Selective Plating of Escherichia coli with bla Plasmids: Carbenicillin (100 µg/mL) selects for beta-lactamase-bearing transformants on rich 2xYT agar.

Transformation Recovery and Colony Isolation: Supports robust outgrowth and discrete colony formation after heat-shock or electroporation.

Plasmid Maintenance and Propagation: Sustains high-copy plasmids under beta-lactam selection for reliable miniprep/maxiprep starter colonies.

Reduced Basal Expression in Lac-Based Systems: 2% glucose represses leaky expression prior to induction, improving stability of toxic or burdensome constructs in Escherichia coli.

Clearer Selection with Fewer Satellites: Carbenicillin’s stability minimizes satellite colonies compared to ampicillin, aiding accurate colony picking.

Colony PCR and Clone Screening: Enables rapid verification of recombinant colonies grown under selective pressure.

Protein Expression Strain Maintenance: Maintains plasmid-bearing Escherichia coli prior to induction, limiting unintended expression during pre-culture steps.

Glycerol Stock Preparation: Provides reliable single colonies for long-term archival of validated clones.