Selection of Kan-Resistant Transformants: Plate transformed Escherichia coli to select clones carrying kanamycin-marked plasmids using 2xYT nutrients (1.6% tryptone, 1.0% yeast extract, 0.5% NaCl) and 50µg/mL kanamycin in 1.5% agar.

Colony Picking and Screening: Isolate individual colonies for plasmid verification (mini-prep, restriction analysis, colony PCR, sequencing) from robust 2xYT growth with kanamycin selection.

Strain Maintenance and Streaking for Purity: Maintain and purify kanamycin-resistant Escherichia coli strains by streaking on selective 2xYT agar plates.

CFU Enumeration of Resistant Cells: Quantify viable counts of kan-marked cells following transformation, conjugation, or mutagenesis on selective 2xYT agar.

Recombinant Plasmid and Vector Propagation: Maintain plasmids carrying kanamycin resistance (e.g., expression or cloning vectors) in Escherichia coli prior to liquid culture scale-up.

Conjugation and Transconjugant Selection: Select kanamycin-resistant transconjugants after bacterial mating on nutrient-rich 2xYT agar.

Transposon Mutagenesis Selection: Recover and select Tn5/Tn-derived kanamycin insertions in Escherichia coli on 2xYT agar plates.

Library Plating and Screening: Plate and screen genomic, plasmid, or CRISPR libraries with kanamycin markers for high colony counts and even growth.

Phage/Phagemid Work (Overlay Base): Use as base plates for top-agar overlays when titering or rescuing kan-marked phagemids in Escherichia coli.