Selection of AmpR Plasmid Transformants: Plating transformed Escherichia coli for selection of ampicillin-resistant colonies on rich 2×YT with 100 µg/mL ampicillin.

High-Density Colony Screening: Large 245 mm plates for library plating, replica plating, and colony lifts from AmpR E. coli clones.

Colony PCR and Sequencing Prep: Picking AmpR colonies from rich glucose-supplemented plates to verify inserts by PCR or Sanger sequencing.

Clone Maintenance and Streaking: Routine streaking and propagation of AmpR E. coli strains on a nutrient-rich, glucose-containing medium.

Plasmid Library Construction: Plating and screening of plasmid libraries requiring ampicillin selection on 2×YT agar.

CFU Enumeration of Transformants: Quantifying transformation efficiency for AmpR constructs on large-format plates.

Blue-White Screening (with Supplements): Color screening of AmpR E. coli when supplemented with X-gal/IPTG on these plates.

Pre-Expression Clone Handling: Maintaining lac/T7-based expression clones; glucose helps minimize leaky expression prior to induction.

Phage and Display Workflows: Plating AmpR host E. coli for phage display or M13 workflows where host selection with ampicillin is required.