Selection of Ampicillin-Resistant Transformants: Plating transformed E. coli to isolate colonies carrying Amp-selectable plasmids.

Plasmid Cloning and Maintenance: Maintaining recombinant plasmids in E. coli with 100 µg/mL ampicillin selection.

Colony Screening and Picking: Growing well-isolated colonies on rich 2xYT agar with 2% glucose for downstream analysis.

Colony PCR Templates: Generating robust colonies for rapid PCR-based insert verification.

Phage Display/Phagemid Workflows: Plating phagemid-containing E. coli; glucose helps repress lac-based promoters during selection.

Protein Expression Workflows (Pre-culture Selection): Selecting correct E. coli clones on plates prior to induction in liquid culture.

Strain Maintenance and Archiving: Streaking and short-term storage of recombinant E. coli under Amp selection.

Library Construction and Screening: Selecting diverse cloning libraries maintained on Amp-resistant E. coli backgrounds.

Transformation Efficiency Assessment: Quantifying colony-forming units after chemical or electroporation transformation.

Plasmid Stability Checks: Monitoring retention of Amp-selectable plasmids in E. coli colonies on rich selective agar.