Selection of Transformants: Plating of Escherichia coli carrying kanamycin-resistant plasmids to recover positive colonies after chemical or electroporation transformation (30 µg/mL kanamycin selection).

Colony Screening and Picking: Growth of recombinant Escherichia coli colonies for downstream PCR screening, restriction analysis, or sequencing of kanamycin-selectable constructs.

Plasmid Maintenance: Routine streaking and passaging of kanamycin-resistant plasmid-bearing Escherichia coli to preserve construct stability on solid rich medium.

Starter Culture Preparation: Isolating single colonies of expression strains (kanR) of Escherichia coli for inoculation of liquid cultures used in recombinant protein production.

Library Plating: Selection and enumeration of kanamycin-marked libraries (e.g., variant, gRNA, or DNA assembly libraries) in Escherichia coli.

CRISPR/Cas Workflows: Recovery of edited Escherichia coli carrying kanamycin-selectable editing plasmids or donor cassettes.

Transposon Mutagenesis: Selection of Escherichia coli mutants harboring kanamycin-resistant transposon insertions for mapping and phenotype screening.

Phage-Related Host Preparation: Plating kanamycin-marked host strains of Escherichia coli used in M13/phage-display workflows prior to infection or rescue.

Quality Control: Monitoring culture purity and verifying plasmid retention in kanamycin-resistant Escherichia coli stocks.