Cloning and Selection of Plasmid-Bearing E. coli: Enriched 2xYT agar with 50µg/mL carbenicillin supports rapid growth and antibiotic selection of transformants.

High-Copy Plasmid Amplification: 2xYT (1.6% tryptone, 1.0% yeast extract, 0.5% NaCl, 1.5% agar) promotes robust colony growth for high plasmid yield.

Leakage Suppression for Toxic Gene Constructs: 2.0% glucose reduces basal expression from lac/ara-inducible systems via catabolite repression during cloning.

Transformation Plating and Colony Isolation: Enables recovery and isolation of antibiotic-resistant colonies after chemical or electroporation transformation.

Colony Screening and Picking: Stable carbenicillin selection minimizes satellite colonies, facilitating accurate colony PCR and miniprep workflows.

Strain Maintenance and Passaging: Preserves plasmid-bearing E. coli strains on selective plates for short-term storage and routine subculture.

Expression Plasmid Maintenance Pre-Induction: Glucose-containing selective plates maintain constructs prior to induction in liquid culture.

Library Construction and Screening: Supports growth and selection of combinatorial or mutagenesis libraries in E. coli.

Phagemid/Helper Phage Workflows: Rich medium with carbenicillin maintains phagemid selection during plaque or colony-based procedures.

CFU Enumeration and Titering: Used to quantify viable transformants under β-lactam selection.