Selective Plating of Transformants: Growth and selection of Escherichia coli carrying bla (ampicillin/carbenicillin)–resistance plasmids at 50 µg/mL carbenicillin.

Cloning and Subcloning Workflows: Isolation of single colonies on rich 2×YT agar for downstream plasmid construction and verification.

Colony Screening: Picking colonies for colony PCR, restriction analysis, or sequencing to confirm inserts.

Starter Culture Preparation: Selecting individual colonies to inoculate liquid media for minipreps, midipreps, and maxipreps.

Plasmid Maintenance: Stable maintenance of bla-marked vectors in E. coli cloning and expression strains.

Protein Expression Workflows: Pre-induction plating of E. coli expression strains (e.g., BL21 derivatives) to verify plasmid integrity before culture scale-up.

Library Construction and Screening: Selection of combinatorial DNA libraries in E. coli on carbenicillin to retain vector backbones.

Reduced Satellite Colonies: Use of carbenicillin minimizes satellite colony formation compared to ampicillin for clearer colony selection.

Strain Preservation and Verification: Streaking and re-plating of glycerol stock isolates to confirm phenotype and plasmid retention.

M13/Phage-Related Host Maintenance: Culturing plasmid-bearing E. coli hosts on rich 2×YT agar when bla selection is required for phage workflows.