Antibiotic Selection of Transformants: Selects Escherichia coli colonies harboring AmpR (bla) plasmids on 2X YT agar with 100 µg/mL ampicillin.

Colony Isolation and Maintenance: Maintains stable Amp-resistant Escherichia coli strains for routine passaging and storage.

Blue-White Cloning Screens: Enables α-complementation screening when supplemented with X-gal/IPTG for insert verification in Escherichia coli.

Colony PCR Screening: Provides selective growth for rapid colony picking and PCR-based insert confirmation.

Plasmid Library Plating: Supports high-efficiency plating of diverse plasmid, gRNA, or mutagenesis libraries under Amp selection.

Phage Display Workflows: Used with Amp-selected Escherichia coli hosts for M13 phage display plating and enrichment.

Pre-Expression Clone Validation: Screens and validates expression clones before liquid culture induction.

Plasmid Stability and Copy-Number Checks: Assesses clone stability and relative copy-number via colony morphology under selection.

CRISPR/Cas Clone Selection: Selects edited Escherichia coli clones carrying Amp-marked editing or helper plasmids.

Colony Picking for Miniprep/Sanger: Provides robust growth for picking single colonies for miniprep and sequencing.