Selection of Ampicillin-Resistant Transformants: Isolation of recombinant E. coli colonies carrying AmpR plasmids on rich 2×YT agar.

Cloning and Plasmid Maintenance: Routine growth and streaking of plasmid-bearing E. coli strains for stable clone preservation.

Colony Screening and PCR: Picking colonies for colony PCR, restriction analysis, or sequencing workflows.

Plasmid Amplification Workflows: Starting single colonies for miniprep/midiprep/maxiprep culture inoculations.

Protein Expression Strain Selection: Maintaining AmpR expression plasmids in E. coli prior to induction in liquid media.

Blue-White Screening (with X-gal/IPTG): Alpha-complementation screening of inserts on Amp plates when supplemented with X-gal and IPTG.

Library Construction and Maintenance: Selection and propagation of plasmid and mutagenesis libraries in E. coli.

Replica Plating and Verification: Phenotypic verification of AmpR and strain authentication by replica plating.