Selection of Carbenicillin-Resistant Transformants: Isolates AmpR plasmid-bearing Escherichia coli colonies using 100 µg/mL carbenicillin.

Colony Screening and Picking: Supports clear, well-separated colony growth on rich 2×YT agar for downstream cloning workflows.

Library Plating and Transformation Efficiency: Quantifies transformants and recovers diverse libraries under beta-lactam selection.

CFU Enumeration and Contamination Checks: Determines viable counts of recombinant E. coli while suppressing non-resistant backgrounds.

Maintenance of Recombinant Strains: Streaks and restreaks AmpR plasmid-containing E. coli for short-term storage and genotype preservation.

Reduced Leaky Expression with Glucose: 2% glucose represses lac/T7-lac promoters to stabilize inserts, aiding cloning of toxic genes.

Pre-Induction Preparation for Expression: Grows starter colonies of expression strains (e.g., T7/lac systems) with minimized basal expression.

Plasmid Stability on Solid Medium: Rich 2×YT nutrients (tryptone, yeast extract, NaCl) support robust colony growth while maintaining selection.

Screening of Ligation and Assembly Reactions: Plates Gibson/Golden Gate products to rapidly identify correct recombinant E. coli clones.