Selection of Ampicillin-Resistant Transformants: Isolates recombinant E. coli carrying bla (AmpR) plasmids at 100 µg/mL ampicillin.

Cloning and Plasmid Maintenance: Supports robust growth in 2×YT for stable propagation of plasmid-bearing E. coli strains.

High-Copy Plasmid Amplification: Enriches plasmid yield from colonies prior to minipreps due to nutrient-rich 2×YT.

Colony Screening/Picking: Enables rapid colony isolation for downstream colony PCR, sequencing, or re-streaking.

Blue-White Screening (with X-gal/IPTG): Facilitates α-complementation-based cloning when supplemented appropriately.

Expression Construct Pre-Screening: Screens antibiotic-selectable expression plasmids in E. coli before induction in liquid culture.

Library Construction and Maintenance: Maintains ampicillin-selectable genomic/cDNA or mutagenesis libraries in E. coli.

Transformation Efficiency Testing: Benchmarks competent cell performance using AmpR plasmid selection.

Phagemid Workflows: Selects AmpR phagemids in 2×YT for M13-based phage/phagemid applications.

CRISPR Plasmid Selection: Maintains ampicillin-selectable CRISPR/Cas plasmids in E. coli workflows.