Dual-Antibiotic Plasmid Selection: Co-selection of plasmids carrying beta-lactamase and tetracycline-resistance markers in Escherichia coli at 100 µg/mL carbenicillin and 20 µg/mL tetracycline.

Stable Clone Maintenance: Maintenance of recombinant strains with reduced satellite colonies due to carbenicillin’s improved stability over ampicillin on rich 2×YT agar.

Co-Transformation Workflows: Selection and maintenance of multi-plasmid Escherichia coli strains requiring concurrent carbenicillin and tetracycline resistance.

Repression of Leaky Expression: 2% glucose supports catabolite repression to minimize basal expression from lac/T7 promoters during cloning and propagation steps.

High-Density Colony Growth: Rich 2×YT formulation (1.6% tryptone, 1.0% yeast extract, 0.5% NaCl) promotes robust colony formation for efficient clone picking and screening.

Phage Display and M13 Work: Plating and titering filamentous phage libraries on F′ Escherichia coli hosts with dual antibiotic selection.

Library Construction and Screening: Growth and selection of combinatorial libraries, mutagenesis libraries, and CRISPR plasmid assemblies requiring strong selection pressure.

Gibson/Golden Gate Cloning Recovery: Post-assembly selection of transformants under carbenicillin/tetracycline to enrich correct constructs.

Bacterial Two-Hybrid and Expression Systems: Support for systems using separate regulatory and reporter plasmids maintained by carbenicillin and tetracycline.

Colony PCR and Sequencing Prep: Reliable colony growth for downstream verification (PCR, miniprep, and Sanger/NGS) from selected clones.