Plasmid Selection and Maintenance: Selection of beta-lactam–resistant plasmids in E. coli using 100 µg/mL carbenicillin on a rich 2xYT base (1.6% Tryptone, 1.0% Yeast Extract, 0.5% NaCl, 1.5% Agar, 2.0% Glucose).

Bacterial Transformation Recovery and Plating: Efficient outgrowth and colony formation of transformed E. coli with AmpR/CarbR vectors.

High-Density Library Plating: Robust growth for combinatorial libraries and cloning workflows enabled by nutrient-rich 2xYT and 2.0% glucose.

Protein Expression Plasmid Maintenance (Pre-Induction): Glucose-mediated catabolite repression helps minimize leaky expression of toxic or burdensome genes in E. coli.

Colony Screening and Picking Automation: 86x128 mm format supports robotic colony picking, replica plating, and high-throughput workflows.

Gibson/Golden Gate/TA Cloning Selection: Reliable antibiotic selection for constructs assembled into Amp/Carb-resistant backbones in E. coli.

Phage Display and M13 Workflows: Host selection for phage-related applications when helper or display plasmids carry beta-lactam markers in E. coli.

CRISPR/Cas Plasmid Work: Maintenance and selection of CRISPR editing and interference plasmids with beta-lactam resistance in E. coli.

Blue–White Screening (with Additives): Compatible with X-gal/IPTG supplementation for alpha-complementation on carbenicillin plates.