Bacterial Culture: Supports robust growth of E. coli and related enteric bacteria for routine propagation.

Plasmid DNA Amplification: Provides high cell density for increased plasmid yield from overnight cultures.

Recombinant Protein Expression: Used for expression workflows in E. coli (e.g., T7/BL21 strains) due to richer nutrient content.

Phage Propagation: Facilitates M13 and filamentous phage amplification, including phage display applications.

Transformation Recovery: Serves as outgrowth medium after chemical or electroporation transformation of E. coli.

Antibiotic Selection Cultures: Employed with appropriate antibiotics to select and maintain recombinant E. coli strains.

Starter and Scale-Up Cultures: Used for inoculating seed cultures and scaling to larger volumes or bioreactors.

Library Construction and Screening: Supports propagation of plasmid, cDNA, and genomic libraries in E. coli.

Protein Purification Workflows: Produces dense cultures for downstream lysis and purification of expressed proteins.

General Cloning Workflows: Suitable for routine subcloning, colony picking, and maintenance of engineered E. coli strains.