Yeast Transformation Selection: Selects Saccharomyces cerevisiae transformants carrying G418/kanamycin resistance (e.g., KanMX) after genomic integration or plasmid uptake.

Plasmid/Genomic Maintenance: Maintains yeast strains with G418-resistant plasmids or integrated cassettes under continuous antibiotic pressure.

Colony Isolation and Titering: Isolates single yeast colonies and determines colony-forming units under G418 selection.

Screening Recombinants: Enables colony PCR or phenotype-based screening of correctly edited yeast clones on selective plates.

Replica Plating: Facilitates high-throughput replica plating to compare growth of resistant versus non-resistant colonies.

Genetic Crosses and Segregant Analysis: Tracks inheritance of G418-resistance markers in tetrads and meiotic segregants.

Strain Verification and Stability Testing: Confirms retention and stability of G418-resistance markers over passages.

Contamination Control: Suppresses growth of non-resistant yeast and many Escherichia coli or Bacillus contaminants during yeast culture handling.

Library Selections: Supports selection of barcoded or pooled yeast libraries using G418 marker enrichment.

Short-Term Stocking: Provides a sterile, ready-to-use medium for short-term storage of selected yeast strains on plates.