Yeast Transformant Selection: Selects for G418-resistant colonies (kanMX) in Saccharomyces and other yeasts on rich YPD.

Plasmid Maintenance in Yeast: Maintains G418-selectable plasmids in engineered yeast strains.

Gene Knockout/Integration Screening: Recovers colonies with kanMX cassette integrations after homologous recombination.

Clone Isolation and Colony Purification: Streaking and restreaking to purify resistant yeast clones under G418 pressure.

Strain Construction and Mating Selections: Selects diploids or segregants retaining the kanMX/G418 marker during crossing and sporulation workflows.

Library Propagation: Maintenance and amplification of kanMX-marked yeast collections (e.g., deletion libraries).

Protein Expression Strain Selection: Ensures retention of G418-selectable constructs in yeast prior to induction or assays.

Contamination Control: Suppresses non-resistant microbes, reducing background from common lab bacteria such as Escherichia coli.