Selection of G418‑Resistant Yeast Transformants: Isolates Saccharomyces cerevisiae and other yeasts carrying KanMX/neo cassettes by growth on YPD (2.0% peptone, 1.0% yeast extract, 2.0% glucose, 1.5% agar) with 500 µg/mL Geneticin.

Plasmid and Genomic Integration Maintenance: Maintains selectable pressure for episomal or integrated constructs conferring G418 resistance in recombinant yeast strains.

Colony Screening and Clonal Isolation: Enables picking and expansion of individual G418‑resistant yeast colonies for downstream analyses.

CRISPR/Cas9 Genome Editing Workflows: Selects edited yeast carrying KanMX/neo selectable markers following genome modification.

Quantification of Transformation Efficiency: Supports colony counting of G418‑resistant transformants to assess protocol and reagent performance.

Yeast Library and Pooled Screens: Applies G418 selection to maintain barcoded or pooled mutant/ORF libraries in yeast.

Strain Preservation and Routine Propagation: Sustains engineered yeast phenotypes requiring continuous G418 selection during passaging.

Two‑Hybrid and Reporter Assays (Selectable Marker Maintenance): Keeps selection for constructs using KanMX/neo resistance in yeast functional assays.

Mating/Sporulation Progeny Selection: Selects desired meiotic segregants retaining G418‑resistance markers after crossing.

Method Development and QC: Serves as a standardized selective medium to benchmark lot consistency and selection stringency for G418‑based workflows.