Selection of NAT-Resistant Yeast Transformants: Isolation of clones carrying nat1/natMX markers after transformation, integration, or plasmid uptake.

Spheroplast Recovery and Plating: Osmotic stabilization with 1M sorbitol for efficient recovery and colony formation of yeast spheroplasts.

CRISPR/Cas9 Genome Editing in Yeast: Selection of edited clones using nourseothricin resistance cassettes in Saccharomyces and nonconventional yeasts.

Candida albicans SAT1-Flipper Workflows: Selection and recycling of SAT1/nat1 markers during targeted gene manipulations.

Plasmid Maintenance and Strain Construction: Stable propagation of yeast strains harboring nat-marked plasmids or genomic insertions.

Osmo-Sensitive Mutant Handling: Growth and phenotyping of osmo-fragile yeast mutants requiring sorbitol for cell wall support.

Library and Transformation Efficiency Screens: High-throughput plating of mutagenesis or expression libraries under nourseothricin selection.

Bacterial Suppression During Yeast Workflows: Inhibition of clonNAT-sensitive contaminants such as Escherichia coli and Bacillus subtilis while selecting yeast.