Kanamycin-Resistant Clone Selection: Isolation of transformants harboring kanamycin-selectable plasmids in Escherichia coli (50 µg/mL kanamycin).

Transformation Plating and Library Screening: Recovery and screening of colonies after chemical or electroporation-based DNA uptake.

Colony Isolation and Strain Maintenance: Streaking for single colonies and routine passage of kanamycin-marked strains on rich 2×YT medium.

Colony PCR and Insert Verification: Picking colonies for PCR screening, restriction analysis, or sequencing of cloned inserts.

CFU Enumeration and Competent Cell QC: Determining transformation efficiency and viable counts under kanamycin selection.

Plasmid Propagation for Miniprep Starters: Selecting stable starters for overnight cultures prior to DNA purification.

Recombinant Protein Workflow Prep: Glucose represses basal expression from lac/T7-lac promoters to stabilize toxic constructs before induction in kanamycin-marked vectors.

Bacterial Genetics and Recombineering: Selection of kanamycin-marked allelic replacements or CRISPR edits in Escherichia coli and related enterics.

Maintenance of KanR Shuttle Vectors in Other Gram-Negatives: Support for kanamycin-selectable plasmids in organisms such as Salmonella enterica when appropriate.