Yeast Transformant Selection: Selects Saccharomyces cerevisiae transformants carrying kanMX/neo resistance on rich YPD with 1000 µg/mL Geneticin G418.

Genomic Integration Screening: Identifies successful integrations marked with kanMX by robust growth on G418-containing YPD.

Plasmid Maintenance: Maintains yeast plasmids bearing kanamycin/neo resistance cassettes through continual G418 selection.

CRISPR/Cas9 Editing Workflows: Enriches edited yeast clones using kanMX selectable markers after genome engineering.

Yeast Deletion Library Handling: Preserves and pins kanMX-marked deletion mutants during high-throughput screening on YPD + G418.

Replica Plating and Colony Purification: Confirms resistance and purifies single colonies under G418 selection pressure.

Mating, Sporulation, and Tetrad Dissection Follow-up: Retains kanMX-marked alleles post-meiosis by selecting G418-resistant progeny.

Two-Hybrid and Interaction Assays: Supports selection of reporter strains or constructs carrying kanMX in rich medium.