Selection of G418-Resistant Yeast Transformants: Isolates yeast clones carrying kanMX or related G418-resistance markers following transformation or genomic integration on rich YPD (1.0% YE, 2.0% peptone, 2.0% glucose, 1.5% agar) with 100µg/mL Geneticin.

Maintenance of Recombinant Yeast Strains: Preserves selective pressure to retain plasmids or integrations conferring Geneticin (G418) resistance during routine culturing and passaging.

Colony Isolation and Purification: Enables streaking for single colonies and purification of G418-resistant yeast lines on a nutrient-rich agar base.

Quantification of Transformants (CFU Counting): Supports plating and enumeration of G418-resistant yeast colonies to assess transformation efficiency.

Genomic Integration Screening: Selects for successful homologous recombination events using kanMX-based cassettes by growth on 100µg/mL Geneticin.

Library and Deletion Collection Workflows: Cultures kanMX-marked yeast deletion mutants and pooled libraries under consistent G418 selection.

Replica Plating and Genetic Screens: Facilitates replica plating of yeast onto G418 plates to identify resistant clones and assess genotype–phenotype relationships.

Plasmid Stability Assays: Evaluates retention of G418-selectable plasmids or integrations over passages in a rich growth environment.

Phenotypic Growth Assays Under Selection: Compares growth rates and stress responses of G418-resistant yeast strains on nutrient-rich, selective agar.

Strain Verification and QC: Confirms antibiotic resistance, purity, and viability of engineered yeast stocks before experimental use.