Yeast Transformation Recovery: Provides 1M sorbitol for osmotic stabilization of spheroplasts post-transformation or electroporation.

Selection of Zeocin-Resistant Yeast: 500µg/mL Zeocin® enables efficient selection of Sh ble–bearing transformants in Saccharomyces and Pichia workflows.

Clonal Isolation and CFU Enumeration: Supports colony formation on rich YPD (2.0% Peptone, 1.0% Yeast Extract, 2.0% Glucose, 1.5% Agar) while applying Zeocin® selection.

Plasmid Maintenance in Yeast: Sustains selective pressure for Zeocin®-marked episomal or integrative constructs during propagation.

Screening Recombinant Expression Strains: Enables picking and verification of Zeocin®-resistant clones for protein production (e.g., Pichia pastoris).

CRISPR/Cas9 Editing Workflows: Post-editing selection of edited yeast carrying Zeocin® resistance cassettes.

Spotting and Phenotypic Assays: Comparative growth, MIC, and fitness assays of Zeocin®-resistant versus control strains on rich medium.

Library Selections: Recovery and selection of mutant or gRNA libraries under Zeocin® pressure.

Streaking and Stock Maintenance: Routine streaking to maintain Zeocin®-resistant yeast lines and prevent loss of selectable markers.

Verification of Integration Events: Plate-based confirmation of genomic integration at target loci using Zeocin® selection.