Selection of G418-resistant yeast transformants: Isolates S. cerevisiae and other yeasts carrying kanMX/neo markers after transformation.

Genome integration and knockout screening: Recovers correct homologous recombination events using G418 resistance as a selectable marker.

CRISPR/Cas9 editing selection: Enriches edited yeast cells harboring G418-selectable repair cassettes.

Plasmid maintenance in yeast: Maintains episomal or integrative plasmids with G418 resistance during propagation.

Replica plating for genotype confirmation: Confirms insertion, loss, or segregation of kanMX-linked loci across colonies.

Construction of deletion libraries: Enables selection and expansion of pooled knockout strains carrying G418-selectable modules.

Transformation efficiency quantification: Determines CFU and transformation frequencies on rich selective medium.

Strain purification and counterselection: Eliminates non-transformed or marker-lost cells to stabilize engineered populations.

Mating and sporulation selections: Selects haploids/diploids retaining kanMX markers after genetic crosses.

Heterologous expression strain upkeep: Sustains recombinant yeast expressing target genes linked to G418 resistance.