Yeast Transformation Selection: Selects Saccharomyces cerevisiae transformants carrying hph/hygR markers after plasmid uptake or genomic integration.

Strain Maintenance and Propagation: Maintains hygromycin‑resistant yeast strains and plasmids on rich YPD for robust growth with selection pressure.

CRISPR/Cas9 Genome Editing Workflows: Screens edited yeast colonies using hygromycin‑B resistance cassettes for successful edits or integrations.

Yeast Two‑Hybrid and Display Systems: Selects for reporter or bait/prey constructs that include hygromycin resistance markers.

Genetic Crosses and Mating Assays: Recovers diploids or segregants retaining hygromycin markers after mating and sporulation workflows.

Library Screening and Clone Picking: Enables high‑efficiency colony isolation, titering, and replica plating under antibiotic selection.

Phenotypic Assays on Rich Medium: Assesses growth rates, drug sensitivities, and stress responses of hygR yeast on a nutrient‑rich background.

Contamination Control: Suppresses growth of non‑resistant microbes, including Escherichia coli and Bacillus subtilis, during yeast work.

Plasmid Stability Testing in Yeast: Monitors maintenance of hygR plasmids under continuous selection across passages.