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Y0167

2XYT Broth. 1000mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$46.00

Fill Volume

1000 mL

Y0167

2XYT Broth. 1000mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

2XYT (Yeast extract Tryptone) Broth is a nutrient-rich liquid medium that supports the cultivation of Escherichia coli. It supplies peptides, amino acids, vitamins, and sodium chloride suitable for growth, and is used for maintenance of recombinant strains, plasmid-bearing cultures, and workflows involving filamentous bacteriophages such as M13.

  • 1.6% Tryptone
  • 1.0% Yeast Extract
  • 0.5% Sodium Chloride
  • High-Density Growth of Escherichia coli: Rich medium supports rapid, dense cultures of E. coli for downstream workflows.

  • Plasmid DNA Preparation: Enhances plasmid yield and quality for mini-, midi-, and maxi-preps from E. coli cultures.

  • Recombinant Protein Expression: Provides robust growth conditions for induction-based protein expression in E. coli.

  • Phage Propagation and Phage Display: Supports amplification of M13 and related filamentous phage using E. coli hosts.

  • Antibiotic Selection and Plasmid Maintenance: Facilitates stable selection pressure and maintenance of expression vectors in E. coli.

  • Transformation Recovery/Outgrowth: Promotes rapid recovery of chemically competent or electrocompetent E. coli post-transformation.

  • Starter and Overnight Cultures: Used to generate reliable inocula for scale-up in shakers or fermenters.

  • General Molecular Cloning Workflows: Suitable for routine cloning steps that require vigorous E. coli growth.

  • pH: 7.00 ± 0.10
Storage Temperature
Room Temperature
Shelf Life
730 days
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