High-Density Cultivation of E. coli: Rich medium supports rapid growth and elevated biomass for routine culture.

Plasmid DNA Amplification: Enhances yield in mini-/midi-/maxipreps from E. coli cultures.

Recombinant Protein Expression in E. coli: Supports robust expression in T7 and other bacterial systems.

Phage Display and M13 Propagation: Facilitates phagemid rescue and filamentous phage amplification from E. coli hosts.

Transformation Recovery/Outgrowth: Promotes recovery of E. coli after heat shock or electroporation prior to plating or selection.

Antibiotic Selection and Plasmid Maintenance: Used with appropriate antibiotics to maintain vectors in E. coli.

Starter and Overnight Cultures: Generates vigorous seed cultures for scale-up and fermentation.

Single-Stranded DNA Production: Supports M13-based ssDNA generation from phagemid-bearing E. coli.

Glycerol Stock Preparation: Produces healthy E. coli cultures for long-term cryostorage.

Library Construction and Cloning Workflows: Enables reliable growth of E. coli for screening and selection of genetic libraries.