High-Density Cultivation: Supports rapid, high-yield growth of Escherichia coli for biomass production in rich, glucose-supplemented medium.

Plasmid DNA Production: Enhances plasmid yield for mini/midi/maxi preps by promoting robust E. coli growth.

Recombinant Protein Expression: Enables pre-induction repression of lac-based systems via glucose, improving stability before IPTG induction in E. coli.

Transformation Recovery: Provides gentle, nutrient-rich outgrowth after chemical transformation or electroporation prior to antibiotic selection.

Antibiotic Selection and Maintenance: Sustains overnight cultures and starter cultures of resistant E. coli clones.

Phage/Phagemid Propagation: Supports M13 and filamentous phage amplification and phagemid production in E. coli hosts.

Library Amplification: Promotes growth for cDNA/genomic, phage display, and mutagenesis libraries in E. coli.

Glycerol Stock Preparation: Produces dense, healthy cultures for long-term E. coli strain banking.

Growth Curves and Physiology: Serves as a rich medium for assessing E. coli growth rates and metabolic studies.

Seed Cultures for Scale-Up: Generates robust inocula for fermentation and bioprocess development in E. coli.