Denaturing Polyacrylamide Gel Electrophoresis (Urea-PAGE): Uses 8M urea to denature RNA and single-stranded DNA for high-resolution separation and sequencing gels.

Nucleic Acid Hybridization and Blotting: Employs 8M urea in hybridization/wash buffers to destabilize secondary structures in Northern and Southern blot protocols.

Protein Solubilization and Inclusion Body Processing: Applies 8M urea as a chaotrope to solubilize aggregated or misfolded proteins from recombinant expression.

Affinity Purification Under Denaturing Conditions: Utilizes 8M urea with Ni-NTA/IMAC workflows to purify His-tagged proteins when native folding prevents binding or solubility.

Protein Refolding Studies: Initiates unfolding in 8M urea followed by stepwise dilution or dialysis to optimize refolding yields and activity.

Isoelectric Focusing and 2D Electrophoresis: Incorporates 8M urea in sample/rehydration buffers to disrupt protein complexes and improve focusing.

Mass Spectrometry Sample Prep: Uses 8M urea to denature proteins prior to reduction/alkylation and protease digestion for proteomics.

RNA Structure and Enzymology: Employs 8M urea to linearize RNA for structure probing, mapping, and accurate size estimation.

Disruption of Macromolecular Interactions: Applies 8M urea to dissociate protein–protein and protein–nucleic acid complexes for analytical studies.

Membrane Protein Extraction: Combines 8M urea with detergents to aid solubilization of hydrophobic and membrane-associated proteins.