High-Density Bacterial Cultivation: Supports dense growth of Escherichia coli and related strains using rich nutrients and phosphate buffering.

Recombinant Protein Expression: Enhances protein yields in Escherichia coli shake flasks and bioreactors due to rich peptides, vitamins, and glycerol carbon source.

Plasmid DNA Production: Maximizes plasmid yield from Escherichia coli cultures for mini-, midi-, and maxi-preps.

Overnight Starter Cultures: Generates robust inocula for scale-up and fermentation with stable pH from phosphate buffering.

Phage Work: Used for M13/phage display propagation and amplification in Escherichia coli.

Post-Transformation Outgrowth: Facilitates rapid recovery of transformed Escherichia coli prior to antibiotic selection.

Glycerol Stock Preparation: Produces high-density cultures suitable for long-term archival stocks of Escherichia coli and other bacteria.

Maintenance of Recombinant Strains: Sustains antibiotic-selected Escherichia coli strains for cloning and expression workflows.