High-Density Cultivation: Supports high-cell-density growth of recombinant Escherichia coli due to elevated tryptone, yeast extract, glycerol, and phosphate buffering.

Kanamycin-Selective Growth: Maintains plasmid selection at 25 µg/mL kanamycin sulfate for stable propagation of Kan^R strains.

Plasmid DNA Production: Enables robust mini/midi/maxi-prep culture growth for high-yield plasmid amplification in Escherichia coli.

Protein Overexpression: Provides nutrient-rich, buffered medium for IPTG- or auto-regulated expression workflows in Escherichia coli carrying Kan^R vectors.

Starter Cultures and Scale-Up: Used for overnight/starter cultures and inoculum build-up prior to fermenter or bioreactor runs.

Post-Transformation Outgrowth: Supports recovery and expansion of kanamycin-resistant transformants before downstream screening.

Clone Maintenance: Long-term maintenance and passaging of Kan^R recombinant strains with consistent selection pressure.

Glycerol Stock Preparation: Reliable medium for preparing cryogenic stocks of Kan^R Escherichia coli clones.

Library Propagation: Growth of cloning, mutagenesis, or display libraries under kanamycin selection for downstream screening.

Sequencing Prep Cultures: Generates clean, high-density cultures for plasmid prep ahead of Sanger or NGS verification.