High-Density Growth of E. coli: Supports robust aerobic cultivation for increased biomass yield in shake flasks and bioreactors.

Recombinant Protein Expression in E. coli: Enhances cell density for higher protein yields under beta-lactam selection pressure.

Plasmid DNA Production: Promotes high-copy plasmid amplification in E. coli for mini-, midi-, and maxi-preps.

Antibiotic Selection and Maintenance: Maintains plasmids carrying beta-lactam resistance using 100 µg/mL carbenicillin.

Overnight Starter Cultures: Reliable medium for inoculum preparation prior to scale-up.

Library Propagation: Growth and maintenance of plasmid libraries and variant pools in E. coli under selection.

Screening of Transformants: Liquid selection to confirm and expand carbenicillin-resistant E. coli clones.

Seed Cultures for Fermentation: Preparation of high-density seed stocks for downstream fermentation runs.

Phage Display and M13 Workflows: Amplification steps requiring robust E. coli growth with plasmid selection.

Glycerol Stock Preparation: Cultivation of plasmid-bearing E. coli prior to long-term cryopreservation.