Polymerase Chain Reaction (PCR): Provides a concentrated stock to prepare PCR buffers at pH 8.0 for optimal polymerase activity.

Gel Electrophoresis: Used to formulate TAE/TBE and Tris-Glycine buffers for agarose gels and SDS-PAGE/native PAGE running systems.

Nucleic Acid Extraction and TE Buffers: Maintains pH during DNA/RNA extraction and in TE buffer for nucleic acid resuspension and storage.

Restriction Digests and Ligation: Serves as the Tris component in reaction buffers to stabilize pH for endonucleases and ligases.

Western Blotting: Prepares Tris-Glycine transfer buffer and TBS/TBST wash buffers for protein transfer and immunodetection.

Protein Purification: Forms the base of binding, wash, and elution buffers in ion exchange and affinity chromatography; supports dialysis buffers.

Enzyme Assays: Provides a stable pH 8.0 environment for kinetic measurements and activity assays.

Bacterial Cell Lysis and Transformation Buffers: Used in resuspension and lysis buffers for workflows involving E. coli and other bacteria.