Polymerase Chain Reaction (PCR/qPCR): Provides 1M Tris-HCl stock to formulate PCR buffers at optimal pH 7.5 for polymerase activity.

Gel Electrophoresis: Used to prepare Tris-based buffers (e.g., TAE/TBE components and sample buffers) ensuring stable pH during nucleic acid separation.

DNA/RNA Extraction and Purification: Included in lysis, wash, and elution buffers to maintain pH for nucleic acid integrity.

Restriction Enzyme Digests: Supplies the Tris-HCl component of digestion buffers at pH 7.5 for consistent enzyme performance.

Ligation Reactions: Provides the Tris-HCl base for ligase buffers to stabilize pH during DNA end-joining.

TE Buffer Preparation: Diluted from 1M Tris-HCl to make TE (e.g., 10 mM Tris-HCl, pH 7.5) for DNA/RNA storage.

Protein Purification: Used in chromatography and general purification buffers to maintain pH during binding, wash, and elution.

Western Blotting: Forms the Tris component of Tris-glycine running and transfer buffers to preserve protein stability.

Enzyme Assays: Provides a stable pH 7.5 environment for diverse enzymatic reactions and kinetic studies.

General Buffer Preparation: Serves as a 1M Tris-HCl stock for rapid, accurate pH-controlled buffer formulation across workflows.