Polymerase Chain Reaction (PCR): Used in PCR mixes and buffers to maintain alkaline pH optimal for DNA polymerase activity.

Gel Electrophoresis: Component for preparing TBE/TG buffers for DNA and protein electrophoresis, ensuring stable pH during runs.

Protein Purification: Employed in ion exchange and affinity chromatography buffers to stabilize protein charge states and binding.

Enzyme Assays: Provides controlled pH for enzymatic reactions, including alkaline phosphatase and other alkaline-optimized enzymes.

Western Blotting: Used to prepare Tris-glycine transfer buffers and TBS/TBST for membrane transfer and antibody incubations.

Nucleic Acid Buffer Preparation: Stock for making TE and other Tris-based buffers for DNA/RNA extraction, cleanup, and storage.

pH Adjustment of Lysis and Extraction Buffers: Adjusts and maintains alkaline pH in cell lysis, protein extraction, and sample prep solutions.

Plasmid Preparation from Escherichia coli: Used to formulate Tris-based resuspension and wash buffers in alkaline lysis workflows.

ELISA and Immunoassays: Preparation of Tris-based dilution and wash buffers to ensure consistent assay performance.

General Laboratory Buffering Agent: Versatile 1M Tris-HCl stock for preparing working buffers at desired pH by dilution and adjustment.