Polymerase Chain Reaction (PCR): Buffer component (1M Tris-HCl, pH 8.3) to maintain optimal pH for Taq polymerase.

Gel Electrophoresis (SDS-PAGE/Native PAGE): Preparation of Tris-Glycine(-SDS) running buffers (~pH 8.3) and Tris-based gel buffers.

Western Blot Transfer: Tris-Glycine transfer buffer preparation for efficient protein transfer.

Restriction Enzyme Digests: Tris-HCl–based reaction buffers to stabilize enzyme activity.

DNA Ligation and Cloning: Tris-HCl–buffered ligation and cloning reactions for stable pH.

Plasmid Miniprep/Maxiprep: Tris-HCl–based resuspension and wash buffers for Escherichia coli plasmid isolation.

TE Buffer Preparation: Tris-EDTA buffer for DNA/RNA dissolution and long-term storage.

Enzyme Assays: General-purpose buffering to ensure reproducible kinetics near pH 8.

Protein Purification (Chromatography): Tris-HCl buffers for column equilibration, wash, and elution in ion-exchange and affinity workflows.

Cell Lysis and Protein Extraction: Tris-HCl–based lysis buffers to stabilize proteins during extraction.