Selection of Carbenicillin-Resistant Transformants: Isolation of plasmid-bearing Escherichia coli colonies using 100 µg/mL carbenicillin for robust beta-lactam selection.

Plasmid Maintenance and Amplification: Stable propagation of beta-lactamase–based vectors in E. coli with high biomass support from Terrific Broth nutrients.

High-Density Colony Growth for Screening: Enhanced colony size and number on 150 mm plates to facilitate rapid visual screening and picking.

Transformation Recovery and CFU Enumeration: Post-heat shock or electroporation plating to quantify transformants and assess cloning efficiency.

Colony PCR and Sequencing Prep: Reliable source of template DNA from picked E. coli colonies for downstream verification.

Blue-White Screening (with Supplements): α-complementation screening on TB agar when supplemented with IPTG/X-gal for insert verification.

Library Construction and Screening: Plating of mutagenesis, genomic, or cDNA libraries requiring strong growth and carbenicillin selection.

Protein Expression Prescreening: Selecting and expanding recombinant E. coli clones prior to induction in liquid culture.

Replica Plating and Strain Verification: Confirming plasmid retention and antibiotic resistance profiles across multiple plates.

Colony Banking and Stock Preparation: Picking well-isolated colonies for glycerol stocks and inoculation of Terrific Broth starter cultures.