Polymerase Chain Reaction (PCR): Buffering component to maintain pH 8.0 for polymerase activity and DNA stability.

Agarose Gel Electrophoresis (DNA/RNA): Preparation of TE buffer and gel/loading buffers to stabilize nucleic acids during separation.

Protein Gel Electrophoresis (SDS-PAGE/native PAGE): Used in Tris–Glycine running and stacking/resolving gels to maintain consistent pH.

Western Blotting: Component of Tris–Glycine transfer buffers to support efficient protein transfer.

Protein Purification: pH control in ion-exchange and affinity chromatography buffers (e.g., equilibration, wash, elution).

Enzyme Assays: Provides stable pH 8.0 conditions for enzymatic kinetics and activity measurements.

Molecular Cloning: Base for restriction digestion, ligation, and transformation-compatible buffers.

Nucleic Acid Preparation: Formulation of TE (Tris-EDTA) for DNA resuspension, storage, and protection from degradation.

Plasmid DNA Workflows: Buffering in miniprep/maxiprep solutions for plasmid isolation from Escherichia coli cultures.

General Laboratory Buffering: Preparation of Tris-based buffers (e.g., TBS) and pH adjustment for biochemical assays.