Antibiotic Selection in Bacterial Culture: Selects for tetracycline-resistant transformants in E. coli and other bacteria during cloning workflows.

Plasmid Maintenance: Maintains plasmids bearing tet resistance markers in liquid culture and on plates for stable propagation in E. coli.

Preparation of Selective Media: Added to LB/agar and other media to create selective conditions for tet-marked constructs in E. coli, Bacillus subtilis, and Staphylococcus aureus.

Inducible Gene Expression (Tet Systems): Used as the regulatory ligand in Tet-On/Tet-Off systems for controllable gene expression in bacterial and mammalian cell culture.

BAC and Shuttle Vector Propagation: Supports selection and maintenance of tet-marked BACs and shuttle vectors during amplification in E. coli.

Screening and Counterselection: Enables differential growth to isolate desired clones and suppress tetracycline-sensitive background bacteria in mixed cultures.

Library Construction and Cloning: Provides selection pressure for combinatorial libraries and CRISPR/cloning constructs carrying tet resistance in E. coli.