Antibiotic Selection in Bacterial Culture: Used to select and maintain plasmids carrying tetR markers in Escherichia coli and Bacillus subtilis.

Maintenance of Cloning and Expression Plasmids: Ensures retention of tetracycline-resistant vectors during cloning and protein expression workflows.

Preparation of Selective Media: Added to agar plates and liquid broth to create tetracycline-selective conditions for bacterial growth.

Tet-Regulated Gene Expression: Controls TetR/Tet-On/Tet-Off systems in bacteria, yeast, and mammalian cells for inducible transcription.

Antimicrobial Susceptibility Testing: Employed in MIC determination, growth inhibition, and kill-curve assays as a reference tetracycline antibiotic.

Bacterial Genome Engineering and Recombineering: Used as a selectable marker for tet-based cassettes in chromosomal edits and BAC workflows.